mef 2a (Proteintech)
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Structured Review
Proteintech
mef 2a
![YTHDF1 decreases sense-derived and increases antisense-derived viral transcripts. HEK293T cells were treated with or without 60 µM STM2457 for 72 h and then transfected with LTR-1-luc, rtk-luc, HTLV-1 proviral DNA, and ±100 ng FLAG-YTHDF1 expression vector. Cells were collected 72 h post-transfection. ( A ) Cells were lysed in passive lysis buffer and firefly luciferase activity was quantified relative to renilla luciferase. ( B ) RNA was isolated, and qRT-PCR was used to measure sense-derived transcripts ( tax, gag, and env ) and antisense-derived ( hbz ) transcripts. ( C ) Western blot analysis was used to measure viral protein (Env, Tax, p19 [Gag], and Hbz) expression. β-actin was used as a loading control. Relative band intensity was quantified using ImageJ. Protein quantification relative to no YTHDF1 (lane 1; normalized to β-actin) is depicted below each western blot. ( D ) Virus release in the cell supernatant was measured using a p19 Gag ELISA. ( E ) Western blot analysis was used to measure transcription factors SP1, JunD, <t>MEF-2A,</t> and MEF-2C. Antibodies against YTHDF1 and β-actin (loading control) were also used. The YTHDF1 and β-actin blots in panel E are duplicates of those depicted in panel C as they are from the same experiment. Relative band intensity was quantified using ImageJ. Protein quantification relative to no YTHDF1 (lane 1; normalized to β-actin) is depicted below each western blot. Statistical significance was determined using a Student’s t -test; * P ≤ 0.05, ** P ≤ 0.01.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5865/pmc11915865/pmc11915865__jvi.02063-24.f004.jpg)
Mef 2a, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mef 2a/product/Proteintech
Average 93 stars, based on 21 article reviews
Mef 2a, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mef 2a/product/Proteintech
Average 93 stars, based on 21 article reviews
mef 2a - by Bioz Stars,
2026-02
93/100 stars
Images
1) Product Images from "YTHDF1 and YTHDC1 m 6 A reader proteins regulate HTLV-1 tax and hbz activity"
Article Title: YTHDF1 and YTHDC1 m 6 A reader proteins regulate HTLV-1 tax and hbz activity
Journal: Journal of Virology
doi: 10.1128/jvi.02063-24
Figure Legend Snippet: YTHDF1 decreases sense-derived and increases antisense-derived viral transcripts. HEK293T cells were treated with or without 60 µM STM2457 for 72 h and then transfected with LTR-1-luc, rtk-luc, HTLV-1 proviral DNA, and ±100 ng FLAG-YTHDF1 expression vector. Cells were collected 72 h post-transfection. ( A ) Cells were lysed in passive lysis buffer and firefly luciferase activity was quantified relative to renilla luciferase. ( B ) RNA was isolated, and qRT-PCR was used to measure sense-derived transcripts ( tax, gag, and env ) and antisense-derived ( hbz ) transcripts. ( C ) Western blot analysis was used to measure viral protein (Env, Tax, p19 [Gag], and Hbz) expression. β-actin was used as a loading control. Relative band intensity was quantified using ImageJ. Protein quantification relative to no YTHDF1 (lane 1; normalized to β-actin) is depicted below each western blot. ( D ) Virus release in the cell supernatant was measured using a p19 Gag ELISA. ( E ) Western blot analysis was used to measure transcription factors SP1, JunD, MEF-2A, and MEF-2C. Antibodies against YTHDF1 and β-actin (loading control) were also used. The YTHDF1 and β-actin blots in panel E are duplicates of those depicted in panel C as they are from the same experiment. Relative band intensity was quantified using ImageJ. Protein quantification relative to no YTHDF1 (lane 1; normalized to β-actin) is depicted below each western blot. Statistical significance was determined using a Student’s t -test; * P ≤ 0.05, ** P ≤ 0.01.
Techniques Used: Derivative Assay, Transfection, Expressing, Plasmid Preparation, Lysis, Luciferase, Activity Assay, Isolation, Quantitative RT-PCR, Western Blot, Control, Virus, Enzyme-linked Immunosorbent Assay
![Knockdown of YTHDF1 increases sense-derived and decreases antisense-derived viral transcripts. SLB-1 and ATL-ED cells were treated with or without 60 µM STM2457 for 72 h. Cells were then transduced with shControl and shYTHDF1 lentivirus. ( A and D ) Western blot analysis was used to measure viral protein (Env, Tax, p19 [Gag], and Hbz), YTHDF1 and β-actin (loading control). Relative band intensity was quantified using ImageJ. Protein quantification relative to DMSO, shControl (lane 1; normalized to β-actin) is depicted below each western blot. ( B ) Virus release in the cell supernatant (of SLB-1 cells) was measured using a p19 Gag ELISA. ( C and E ) RNA was isolated and qRT-PCR was used to measure the transcript levels of tax , env , gag , and hbz relative to gapdh . ( F ) SLB-1 cells and ( G ) ATL-ED cells were subjected to immunoblot to measure YTHDF1, JunD, SP1, MEF-2A, MEF-2C, and β-actin (loading control). The YTHDF1 and β-actin blots in panels F and G are duplicates of those depicted in panels A and D, respectively, as they are from the same experiment. Relative band intensity was quantified using ImageJ. Protein quantification relative to shControl, DMSO (lane 1; normalized to β-actin) is depicted below each western blot. Statistical significance was determined using a Student’s t -test; * P ≤ 0.05, ** P ≤ 0.01.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5865/pmc11915865/pmc11915865__jvi.02063-24.f005.jpg "... subjected to immunoblot to measure YTHDF1, JunD, SP1, MEF-2A, MEF-2C, and β-actin (loading control). The YTHDF1 and ...")
Figure Legend Snippet: Knockdown of YTHDF1 increases sense-derived and decreases antisense-derived viral transcripts. SLB-1 and ATL-ED cells were treated with or without 60 µM STM2457 for 72 h. Cells were then transduced with shControl and shYTHDF1 lentivirus. ( A and D ) Western blot analysis was used to measure viral protein (Env, Tax, p19 [Gag], and Hbz), YTHDF1 and β-actin (loading control). Relative band intensity was quantified using ImageJ. Protein quantification relative to DMSO, shControl (lane 1; normalized to β-actin) is depicted below each western blot. ( B ) Virus release in the cell supernatant (of SLB-1 cells) was measured using a p19 Gag ELISA. ( C and E ) RNA was isolated and qRT-PCR was used to measure the transcript levels of tax , env , gag , and hbz relative to gapdh . ( F ) SLB-1 cells and ( G ) ATL-ED cells were subjected to immunoblot to measure YTHDF1, JunD, SP1, MEF-2A, MEF-2C, and β-actin (loading control). The YTHDF1 and β-actin blots in panels F and G are duplicates of those depicted in panels A and D, respectively, as they are from the same experiment. Relative band intensity was quantified using ImageJ. Protein quantification relative to shControl, DMSO (lane 1; normalized to β-actin) is depicted below each western blot. Statistical significance was determined using a Student’s t -test; * P ≤ 0.05, ** P ≤ 0.01.
Techniques Used: Knockdown, Derivative Assay, Transduction, Western Blot, Control, Virus, Enzyme-linked Immunosorbent Assay, Isolation, Quantitative RT-PCR
![Figure 5. Mettl3 deficiency causes hypomethylation of the Sox4, Sox11, and <t>Mef2a</t> transcripts in mouse embryonic hearts. A Schematic diagram of the MeRIP-seq analysis used to identify m6A methylation target genes in mouse embryonic hearts. B Volcano plot showing genes corresponding to the differentially m6A-methylated transcripts detected by MeRIP-seq analysis in the hearts of Mettl3-CV KO and control mouse embryos at E10.5-11.5. The abscissa axis is log2 (fold change), and the ordinate axis is [-log10 (adjusted P value)]. The gray dots indicate the genes without differentially methylated m6A peaks, the blue dots indicate the genes with differentially hypomethylated m6A peaks in the Mettl3-CV KO group, and the red dots indicate the genes with differentially hypermethylated m6A peaks in the Mettl3-CV KO group. C Schematic diagram of screening m6A methylation target genes involved in congenital cardiac defects. D MeRIP-qPCR analysis of m6A enrichment in the transcripts of Mef2a, Sox4, Sox11, and Gata5 in the hearts of Mettl3-CV KO and control mouse embryos at E10.5-11.5. n = 3 per group, and each sample was obtained from 15 embryos. The data are presented as the mean ± SEM and were analyzed by using two-way ANOVA followed by Tukey’s test for post hoc comparisons. **, P< 0.01, ***, P< 0.001. ns = not significant.](https://doi-unpaywalled-images-cdn.bioz.com/8088/10__7150_slash_ijbs__100941/10__7150_slash_ijbs__100941____page11_image1.jpg)
